Flow cytometric measurement of DCFH-oxidation f neutrophils in peripheral whole blood, isolated leukocytes and cerebrospinal fluid cells.
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چکیده
منابع مشابه
Flow cytometric immunophenotyping of cerebrospinal fluid specimens.
Flow cytometric immunophenotyping (FCI) is recommended in the evaluation of cerebrospinal fluid (CSF) specimens for hematologic neoplasms. This study reviewed FCI of CSF specimens collected for primary diagnosis (n = 77) and follow-up for known malignancy (n = 153). FCI was positive in 11 (4.8%) of 230 specimens: acute myeloid leukemia, 6; precursor B-acute lymphoblastic leukemia, 2; B-cell lym...
متن کاملAutomated flow cytometric analysis of cerebrospinal fluid.
BACKGROUND Recently, the UF-100 (Sysmex Corporation) flow cytometer was developed to automate urinalysis. We evaluated the use of flow cytometry in the analysis of cerebrospinal fluid (CSF). METHODS UF-100 data were correlated with microscopy and biochemical data for 256 CSF samples. Microbiological analysis was performed in 144 suspected cases of meningitis. RESULTS Good agreement was obta...
متن کاملArchetypal and rearranged sequences of human polyomavirus JC transcription control region in peripheral blood leukocytes and in cerebrospinal fluid.
Two forms of human polyomavirus JC (JCV) genome are known based upon the structure of the transcriptional control region (TCR) of the virus: the archetypal form, which is commonly detected in urine, and the rearranged form, which was first detected in brain tissue from progressive multifocal leukoencephalopathy (PML) patients. The latter actually includes a group of TCR variants that, relative ...
متن کاملImmune cells subpopulations in cerebrospinal fluid and peripheral blood of patients with Aneurysmal Subarachnoid Hemorrhage
BACKGROUND There is growing evidence supporting the role of inflammation in aneurysmal subarachnoid hemorrhage (aSAH) pathophysiology and it is of great interest to elucidate which immune mechanisms are involved. METHODS 12 aSAH patients and 28 healthy controls were enrolled prospectively. We assessed leukocytes subpopulations and their activation status by flow cytometry in cerebrospinal flu...
متن کاملRapid Flow Cytometric Method for Measuring Mitochondrial Membrane Potential, Respiratory Burst Activity, and Intracellular Thiols of Human Whole Blood Leukocytes
Identifying key perturbations of redox regulatory mechanisms may help to understand stress-related disease processes. Currently, flow cytometry allows the functional characterisation of redox changes that occur in different cellular compartments: changes in mitochondrial membrane potential, respiratory burst activity, and intracellular thiol levels are measurable in live white blood cells. Howe...
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ژورنال
عنوان ژورنال: Journal of Nippon Medical School
سال: 1996
ISSN: 0048-0444,1884-0108
DOI: 10.1272/jnms1923.63.192